Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1366810..1367585 Replicon chromosome
Accession NZ_CP043047
Organism Klebsiella pneumoniae strain KLP268

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag FXN60_RS06690 Protein ID WP_004150910.1
Coordinates 1366810..1367295 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag FXN60_RS06695 Protein ID WP_004150912.1
Coordinates 1367292..1367585 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FXN60_RS06655 1361979..1363346 - 1368 WP_004150905.1 replicative DNA helicase -
FXN60_RS06660 1363339..1363722 - 384 WP_004150906.1 hypothetical protein -
FXN60_RS06665 1363722..1364594 - 873 WP_004188557.1 ParA family protein -
FXN60_RS06680 1365200..1365416 - 217 Protein_1278 transposase -
FXN60_RS06685 1365513..1366106 - 594 WP_004188553.1 hypothetical protein -
FXN60_RS06690 1366810..1367295 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
FXN60_RS06695 1367292..1367585 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
FXN60_RS06700 1368230..1369606 + 1377 WP_004217775.1 cystathionine beta-synthase -
FXN60_RS06705 1369617..1370765 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
FXN60_RS06710 1370766..1371677 - 912 WP_004188550.1 LysR family transcriptional regulator -
FXN60_RS06715 1371775..1372377 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 1309790..1367836 58046
flank IS/Tn - - 1365264..1365416 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T136999 WP_004150910.1 NZ_CP043047:c1367295-1366810 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T136999 NZ_CP043047:c1367295-1366810 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT136999 WP_004150912.1 NZ_CP043047:c1367585-1367292 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT136999 NZ_CP043047:c1367585-1367292 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References