Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3805520..3806295 Replicon chromosome
Accession NZ_CP023946
Organism Klebsiella pneumoniae strain FDAARGOS_446

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag CRN69_RS19165 Protein ID WP_004150910.1
Coordinates 3805520..3806005 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag CRN69_RS19170 Protein ID WP_004150912.1
Coordinates 3806002..3806295 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN69_RS19135 3800689..3802056 - 1368 WP_004150905.1 replicative DNA helicase -
CRN69_RS19140 3802049..3802432 - 384 WP_004150906.1 hypothetical protein -
CRN69_RS19145 3802432..3803304 - 873 WP_004188557.1 ParA family protein -
CRN69_RS19155 3803910..3804126 - 217 Protein_3680 transposase -
CRN69_RS19160 3804223..3804816 - 594 WP_004188553.1 hypothetical protein -
CRN69_RS19165 3805520..3806005 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
CRN69_RS19170 3806002..3806295 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
CRN69_RS19175 3806940..3808316 + 1377 WP_004217775.1 cystathionine beta-synthase -
CRN69_RS19180 3808327..3809475 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
CRN69_RS19185 3809476..3810387 - 912 WP_004188550.1 LysR family transcriptional regulator -
CRN69_RS19190 3810485..3811087 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 3747435..3806546 59111
flank IS/Tn - - 3803974..3804126 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T86418 WP_004150910.1 NZ_CP023946:c3806005-3805520 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T86418 NZ_CP023946:c3806005-3805520 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT86418 WP_004150912.1 NZ_CP023946:c3806295-3806002 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT86418 NZ_CP023946:c3806295-3806002 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References