Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4628664..4629439 Replicon chromosome
Accession NZ_CP060744
Organism Klebsiella pneumoniae strain KP120

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag IAI03_RS22660 Protein ID WP_004150910.1
Coordinates 4628664..4629149 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IAI03_RS22665 Protein ID WP_004150912.1
Coordinates 4629146..4629439 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAI03_RS22640 4624211..4625581 - 1371 WP_110212567.1 replicative DNA helicase -
IAI03_RS22645 4625574..4626449 - 876 WP_187475359.1 ParA family protein -
IAI03_RS22650 4627054..4627270 - 217 Protein_4421 transposase -
IAI03_RS22655 4627367..4627960 - 594 WP_004188553.1 hypothetical protein -
IAI03_RS22660 4628664..4629149 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
IAI03_RS22665 4629146..4629439 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IAI03_RS22670 4630084..4631460 + 1377 WP_004217775.1 cystathionine beta-synthase -
IAI03_RS22675 4631471..4632619 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
IAI03_RS22680 4632620..4633531 - 912 WP_004188550.1 LysR family transcriptional regulator -
IAI03_RS22685 4633629..4634231 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4560810..4629691 68881
flank IS/Tn - - 4627118..4627270 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T170926 WP_004150910.1 NZ_CP060744:c4629149-4628664 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T170926 NZ_CP077779:c5267393-5267010 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT170926 WP_004150912.1 NZ_CP060744:c4629439-4629146 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT170926 NZ_CP077779:c5267635-5267393 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References