Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 775159..775934 Replicon chromosome
Accession NZ_CP063759
Organism Klebsiella pneumoniae strain FRPDR

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag IM727_RS03905 Protein ID WP_004150910.1
Coordinates 775449..775934 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IM727_RS03900 Protein ID WP_004150912.1
Coordinates 775159..775452 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM727_RS03880 770367..770969 - 603 WP_062954968.1 short chain dehydrogenase -
IM727_RS03885 771067..771978 + 912 WP_004188550.1 LysR family transcriptional regulator -
IM727_RS03890 771979..773127 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
IM727_RS03895 773138..774514 - 1377 WP_004217775.1 cystathionine beta-synthase -
IM727_RS03900 775159..775452 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IM727_RS03905 775449..775934 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
IM727_RS03910 776638..777231 + 594 WP_004188553.1 hypothetical protein -
IM727_RS03915 777328..777544 + 217 Protein_770 transposase -
IM727_RS03920 778150..779022 + 873 WP_004188557.1 ParA family protein -
IM727_RS03925 779022..779405 + 384 WP_004150906.1 hypothetical protein -
IM727_RS03930 779398..780765 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 777328..777480 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T178733 WP_004150910.1 NZ_CP063759:775449-775934 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T178733 NZ_CP084222:2159172-2159429 [Arsenophonus apicola]
ATGAAATTAATTTTTACCGAACAATCTTGGAACGATTACCTTTATTGGCAGCAGATAGATAAAAAGGTAATTAAACGTAT
TAATGAATTAATTAAAGATATTAAAAGAGAACTTTTTTCTGGTATCGGTAAGCCCGAGCCTTTAAAACATAATTTAACCG
GTTTTTGGTCGCGCAGAATAACTGAGGAACACCGTTTAATTTACCGGTTAACAGATAAGAGCTTAGAGATAGCTTCATGT
CGTTATCACTATAATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT178733 WP_004150912.1 NZ_CP063759:775159-775452 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT178733 NZ_CP084222:2158924-2159175 [Arsenophonus apicola]
ATGAAAACAGTCAATTATACCGATGCAAGACAAAACTTAACCTCTATCATGAATGAAGCGATAGATGATCGGATCCCAAT
TTTAATCACTAGACAAAAAGGGATCCCTTGCGTTTTGTTATCACATGATGAATACCAATCTTTAGAAGAGACGGCCTACC
TGCTGCGCTCGCCAGCTAATGCTCGTCATCTATTGGATTCAATTGATGAACTCAATGCCGGTAAAGGAATTGAAAGAGAT
ATTCAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References