Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739025..739800 Replicon chromosome
Accession NZ_CP090931
Organism Klebsiella pneumoniae strain ONE259

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I7858_RS03690 Protein ID WP_004150910.1
Coordinates 739315..739800 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I7858_RS03685 Protein ID WP_004150912.1
Coordinates 739025..739318 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I7858_RS03665 (I7858_03665) 734234..734836 - 603 WP_002916735.1 short chain dehydrogenase -
I7858_RS03670 (I7858_03670) 734934..735845 + 912 WP_004188550.1 LysR family transcriptional regulator -
I7858_RS03675 (I7858_03675) 735846..736994 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I7858_RS03680 (I7858_03680) 737005..738380 - 1376 Protein_723 cystathionine beta-synthase -
I7858_RS03685 (I7858_03685) 739025..739318 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I7858_RS03690 (I7858_03690) 739315..739800 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I7858_RS03695 (I7858_03695) 740504..741097 + 594 WP_004188553.1 hypothetical protein -
I7858_RS03700 (I7858_03700) 741194..741410 + 217 Protein_727 transposase -
I7858_RS03705 (I7858_03705) 742016..742888 + 873 WP_004188557.1 ParA family protein -
I7858_RS03710 (I7858_03710) 742888..743271 + 384 WP_004150906.1 hypothetical protein -
I7858_RS03715 (I7858_03715) 743264..744631 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 741194..741346 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T232189 WP_004150910.1 NZ_CP090931:739315-739800 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T232189 NZ_CP127274:363475-363777 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT232189 WP_004150912.1 NZ_CP090931:739025-739318 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT232189 NZ_CP127274:363251-363478 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References