Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4302904..4303679 Replicon chromosome
Accession NZ_CP061965
Organism Klebsiella pneumoniae strain LZK001

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag H4I72_RS20780 Protein ID WP_009308645.1
Coordinates 4302904..4303389 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag H4I72_RS20785 Protein ID WP_004150912.1
Coordinates 4303386..4303679 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I72_RS20760 4299903..4300616 + 714 WP_002916694.1 DUF554 domain-containing protein -
H4I72_RS20770 4301294..4301510 - 217 Protein_4073 transposase -
H4I72_RS20775 4301607..4302200 - 594 WP_076027112.1 hypothetical protein -
H4I72_RS20780 4302904..4303389 - 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
H4I72_RS20785 4303386..4303679 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
H4I72_RS20790 4304324..4305700 + 1377 WP_072158501.1 cystathionine beta-synthase -
H4I72_RS20795 4305711..4306859 + 1149 WP_076027111.1 PLP-dependent aspartate aminotransferase family protein -
H4I72_RS20800 4306860..4307771 - 912 WP_014906929.1 LysR family transcriptional regulator -
H4I72_RS20805 4307869..4308471 + 603 WP_076027110.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4301358..4301510 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T173309 WP_009308645.1 NZ_CP061965:c4303389-4302904 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T173309 NZ_CP080139:1126968-1127255 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT173309 WP_004150912.1 NZ_CP061965:c4303679-4303386 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT173309 NZ_CP080139:1126729-1126968 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References