Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 731972..732747 Replicon chromosome
Accession NZ_CP075548
Organism Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KI675_RS03625 Protein ID WP_004150910.1
Coordinates 732262..732747 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KI675_RS03620 Protein ID WP_004150912.1
Coordinates 731972..732265 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI675_RS03600 (727180) 727180..727782 - 603 WP_002916735.1 short chain dehydrogenase -
KI675_RS03605 (727880) 727880..728791 + 912 WP_004188550.1 LysR family transcriptional regulator -
KI675_RS03610 (728792) 728792..729940 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KI675_RS03615 (729951) 729951..731327 - 1377 WP_004217775.1 cystathionine beta-synthase -
KI675_RS03620 (731972) 731972..732265 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KI675_RS03625 (732262) 732262..732747 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KI675_RS03630 (733451) 733451..734044 + 594 WP_004188553.1 hypothetical protein -
KI675_RS03635 (734141) 734141..734357 + 217 Protein_714 transposase -
KI675_RS03645 (735035) 735035..735748 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 734141..734293 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T203826 WP_004150910.1 NZ_CP075548:732262-732747 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T203826 NZ_CP100388:1760994-1761515 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTCCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGTGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT203826 WP_004150912.1 NZ_CP075548:731972-732265 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT203826 NZ_CP100388:1760718-1760987 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References