Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 738791..739566 Replicon chromosome
Accession NZ_CP054268
Organism Klebsiella pneumoniae strain 39427

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HPS02_RS05430 Protein ID WP_004150910.1
Coordinates 739081..739566 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HPS02_RS05425 Protein ID WP_004150912.1
Coordinates 738791..739084 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPS02_RS05405 733999..734601 - 603 WP_002916735.1 short chain dehydrogenase -
HPS02_RS05410 734699..735610 + 912 WP_004188550.1 LysR family transcriptional regulator -
HPS02_RS05415 735611..736759 - 1149 WP_087749889.1 PLP-dependent aspartate aminotransferase family protein -
HPS02_RS05420 736770..738146 - 1377 WP_004217775.1 cystathionine beta-synthase -
HPS02_RS05425 738791..739084 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HPS02_RS05430 739081..739566 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HPS02_RS05435 740270..740863 + 594 WP_004188553.1 hypothetical protein -
HPS02_RS05440 740960..741176 + 217 Protein_726 transposase -
HPS02_RS05445 741782..742654 + 873 WP_004188557.1 ParA family protein -
HPS02_RS05450 742654..743037 + 384 WP_004150906.1 hypothetical protein -
HPS02_RS05455 743030..744397 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 740960..741112 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T159242 WP_004150910.1 NZ_CP054268:739081-739566 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T159242 NZ_CP069990:c2324616-2324314 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT159242 WP_004150912.1 NZ_CP054268:738791-739084 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT159242 NZ_CP069990:c2324278-2323937 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References