Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 769339..770114 Replicon chromosome
Accession NZ_CP118398
Organism Klebsiella pneumoniae strain 2020CK-00223

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag PWK27_RS03900 Protein ID WP_021314147.1
Coordinates 769629..770114 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag PWK27_RS03895 Protein ID WP_004150912.1
Coordinates 769339..769632 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PWK27_RS03875 (PWK27_03875) 764547..765149 - 603 WP_004174410.1 short chain dehydrogenase -
PWK27_RS03880 (PWK27_03880) 765247..766158 + 912 WP_104159329.1 LysR family transcriptional regulator -
PWK27_RS03885 (PWK27_03885) 766159..767307 - 1149 WP_020316731.1 PLP-dependent aspartate aminotransferase family protein -
PWK27_RS03890 (PWK27_03890) 767318..768694 - 1377 WP_004174417.1 pyridoxal-phosphate dependent enzyme -
PWK27_RS03895 (PWK27_03895) 769339..769632 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
PWK27_RS03900 (PWK27_03900) 769629..770114 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
PWK27_RS03905 (PWK27_03905) 770818..771411 + 594 WP_274759606.1 hypothetical protein -
PWK27_RS03910 (PWK27_03910) 771508..771724 + 217 Protein_768 transposase -
PWK27_RS03920 (PWK27_03920) 772239..772952 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 771508..771660 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T273093 WP_021314147.1 NZ_CP118398:769629-770114 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT273093 WP_004150912.1 NZ_CP118398:769339-769632 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References