Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 729402..730177 Replicon chromosome
Accession NZ_CP055293
Organism Klebsiella pneumoniae strain 200

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HWA76_RS03610 Protein ID WP_004150910.1
Coordinates 729692..730177 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HWA76_RS03605 Protein ID WP_004150912.1
Coordinates 729402..729695 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWA76_RS03585 (724610) 724610..725212 - 603 WP_002916735.1 short chain dehydrogenase -
HWA76_RS03590 (725310) 725310..726221 + 912 WP_004188550.1 LysR family transcriptional regulator -
HWA76_RS03595 (726222) 726222..727370 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HWA76_RS03600 (727381) 727381..728757 - 1377 WP_004217775.1 cystathionine beta-synthase -
HWA76_RS03605 (729402) 729402..729695 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HWA76_RS03610 (729692) 729692..730177 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HWA76_RS03615 (730881) 730881..731474 + 594 WP_004188553.1 hypothetical protein -
HWA76_RS03620 (731571) 731571..731787 + 217 Protein_711 transposase -
HWA76_RS03625 (732393) 732393..733265 + 873 WP_004188557.1 ParA family protein -
HWA76_RS03630 (733265) 733265..733648 + 384 WP_004150906.1 hypothetical protein -
HWA76_RS03635 (733641) 733641..735008 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 731571..731723 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T162264 WP_004150910.1 NZ_CP055293:729692-730177 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T162264 NZ_CP071708:2803206-2803508 [Vibrio sp. ABG19]
ATGGCTAAAATAATCTGGACTGAGCCAGCGTTATCTGACCTAGATGATATCGCTGAATACATCGCACTTGAAAAATTTGT
AGCTGCAAAGCAATTGGTTCAAACGATCTTCGCTAAAGTTGAACGTTTAGAAACTTTCCCTGAATCGGGTCGGATTCCGC
CCGAACTAGAACATTTAAGTTATCGAGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGATAAAGTA
TTTATTCTGTTTGTCATGCGTGTTGAGAGAGATTTGCGTAAGTTCCTGCTAAGTAAGGTGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT162264 WP_004150912.1 NZ_CP055293:729402-729695 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT162264 NZ_CP071708:2802961-2803218 [Vibrio sp. ABG19]
ATGAAAGTAGAATTAGTAACATCACTTAAGCGCCAAGCCACTAAGATCCTTGCCGATCTTCACGATACCAAAGAACCAGT
ATTAATTACCGAGCATGGTAAACCGTCTGCATATTTGGTAGATGTTGATGATTACGAGTTTATGCAAAACCGTTTATCAA
TTCTTGAGGGGATTGCGCGAGGCGAACGTGCACTAACTGATGGTAAAATGGTGAGTCATGAAGAAGCCAAAGACAAAATG
TCAAAATGGCTAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References