Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 729462..730237 Replicon chromosome
Accession NZ_CP082799
Organism Klebsiella pneumoniae strain SCAID TST1-2021 (15/226)

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag K8B70_RS03620 Protein ID WP_009308645.1
Coordinates 729752..730237 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K8B70_RS03615 Protein ID WP_004150912.1
Coordinates 729462..729755 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B70_RS03595 (724670) 724670..725272 - 603 WP_004174410.1 short chain dehydrogenase -
K8B70_RS03600 (725370) 725370..726281 + 912 WP_016947442.1 LysR family transcriptional regulator -
K8B70_RS03605 (726282) 726282..727430 - 1149 WP_016947441.1 PLP-dependent aspartate aminotransferase family protein -
K8B70_RS03610 (727441) 727441..728817 - 1377 WP_016947440.1 cystathionine beta-synthase -
K8B70_RS03615 (729462) 729462..729755 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K8B70_RS03620 (729752) 729752..730237 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
K8B70_RS03625 (730941) 730941..731186 + 246 Protein_713 hypothetical protein -
K8B70_RS03635 (731872) 731872..732306 + 435 Protein_715 hypothetical protein -
K8B70_RS03640 (732403) 732403..732619 + 217 Protein_716 transposase -
K8B70_RS03650 (733297) 733297..734010 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 732403..732555 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T215122 WP_009308645.1 NZ_CP082799:729752-730237 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T215122 NZ_CP110483:2314759-2315109 [Weizmannia coagulans]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCGATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAAAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT215122 WP_004150912.1 NZ_CP082799:729462-729755 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT215122 NZ_CP110483:2314476-2314754 [Weizmannia coagulans]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAATTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACAATGAGGCGTGGTTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References