Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 740844..741619 Replicon chromosome
Accession NZ_CP083784
Organism Klebsiella pneumoniae strain KSB2_6C isolate KSB2_6C

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LD127_RS03670 Protein ID WP_004150910.1
Coordinates 741134..741619 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LD127_RS03665 Protein ID WP_004150912.1
Coordinates 740844..741137 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD127_RS03645 (736052) 736052..736654 - 603 WP_002916735.1 short chain dehydrogenase -
LD127_RS03650 (736752) 736752..737663 + 912 WP_004188550.1 LysR family transcriptional regulator -
LD127_RS03655 (737664) 737664..738812 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LD127_RS03660 (738823) 738823..740199 - 1377 WP_004217775.1 cystathionine beta-synthase -
LD127_RS03665 (740844) 740844..741137 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LD127_RS03670 (741134) 741134..741619 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LD127_RS03675 (742323) 742323..742916 + 594 WP_004188553.1 hypothetical protein -
LD127_RS03680 (743013) 743013..743229 + 217 Protein_724 transposase -
LD127_RS03690 (743907) 743907..744620 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743013..743165 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T217316 WP_004150910.1 NZ_CP083784:741134-741619 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T217316 NZ_CP114149:4576020-4576238 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT217316 WP_004150912.1 NZ_CP083784:740844-741137 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT217316 NZ_CP114149:4575619-4575993 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTACGCCGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCTGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References