Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1285475..1286250 Replicon chromosome
Accession NZ_CP070105
Organism Klebsiella pneumoniae strain FDAARGOS_1314

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag I6K44_RS06360 Protein ID WP_009308645.1
Coordinates 1285475..1285960 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I6K44_RS06365 Protein ID WP_004150912.1
Coordinates 1285957..1286250 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K44_RS06335 1280643..1282010 - 1368 WP_040236167.1 replicative DNA helicase -
I6K44_RS06340 1282003..1282386 - 384 WP_040236166.1 hypothetical protein -
I6K44_RS06345 1282386..1283258 - 873 WP_040236165.1 ParA family protein -
I6K44_RS06350 1283865..1284081 - 217 Protein_1237 transposase -
I6K44_RS06355 1284178..1284771 - 594 WP_004188553.1 hypothetical protein -
I6K44_RS06360 1285475..1285960 - 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
I6K44_RS06365 1285957..1286250 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I6K44_RS06370 1286895..1288271 + 1377 WP_072269297.1 cystathionine beta-synthase -
I6K44_RS06375 1288282..1289430 + 1149 WP_040236164.1 PLP-dependent aspartate aminotransferase family protein -
I6K44_RS06380 1289431..1290342 - 912 WP_004181308.1 LysR family transcriptional regulator -
I6K44_RS06385 1290440..1291042 + 603 WP_032448122.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1226648..1286501 59853
flank IS/Tn - - 1283929..1284081 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T193147 WP_009308645.1 NZ_CP070105:c1285960-1285475 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T193147 NZ_CP092718:c112477-112061 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT193147 WP_004150912.1 NZ_CP070105:c1286250-1285957 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT193147 NZ_CP092718:c112704-112474 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References