Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742073..742848 Replicon chromosome
Accession NZ_CP050342
Organism Klebsiella pneumoniae strain ZRKP03

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HB788_RS03715 Protein ID WP_004150910.1
Coordinates 742363..742848 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HB788_RS03710 Protein ID WP_004150912.1
Coordinates 742073..742366 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB788_RS03690 737281..737883 - 603 WP_062954968.1 short chain dehydrogenase -
HB788_RS03695 737981..738892 + 912 WP_004188550.1 LysR family transcriptional regulator -
HB788_RS03700 738893..740041 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HB788_RS03705 740052..741428 - 1377 WP_004217775.1 cystathionine beta-synthase -
HB788_RS03710 742073..742366 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HB788_RS03715 742363..742848 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HB788_RS03720 743552..744145 + 594 WP_004188553.1 hypothetical protein -
HB788_RS03725 744242..744458 + 217 Protein_732 transposase -
HB788_RS03730 745064..745936 + 873 WP_004188557.1 ParA family protein -
HB788_RS03735 745936..746319 + 384 WP_004150906.1 hypothetical protein -
HB788_RS03740 746312..747679 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744242..744394 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T152641 WP_004150910.1 NZ_CP050342:742363-742848 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T152641 NZ_CP066366:2226102-2226389 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT152641 WP_004150912.1 NZ_CP050342:742073-742366 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT152641 NZ_CP066366:2225863-2226102 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References