Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4943789..4944564 Replicon chromosome
Accession NZ_CP072460
Organism Klebsiella pneumoniae strain K-1L

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag J7V83_RS24310 Protein ID WP_009308645.1
Coordinates 4944079..4944564 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag J7V83_RS24305 Protein ID WP_004150912.1
Coordinates 4943789..4944082 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V83_RS24285 (4938997) 4938997..4939599 - 603 WP_004174410.1 short chain dehydrogenase -
J7V83_RS24290 (4939697) 4939697..4940608 + 912 WP_004188550.1 LysR family transcriptional regulator -
J7V83_RS24295 (4940609) 4940609..4941757 - 1149 WP_020316731.1 PLP-dependent aspartate aminotransferase family protein -
J7V83_RS24300 (4941768) 4941768..4943144 - 1377 WP_074424416.1 pyridoxal-phosphate dependent enzyme -
J7V83_RS24305 (4943789) 4943789..4944082 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
J7V83_RS24310 (4944079) 4944079..4944564 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
J7V83_RS24315 (4945268) 4945268..4945861 + 594 WP_004188553.1 hypothetical protein -
J7V83_RS24320 (4945958) 4945958..4946174 + 217 Protein_4748 transposase -
J7V83_RS24330 (4946852) 4946852..4947565 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4945958..4946110 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T197830 WP_009308645.1 NZ_CP072460:4944079-4944564 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T197830 NZ_CP096759:450844-451233 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT197830 WP_004150912.1 NZ_CP072460:4943789-4944082 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT197830 NZ_CP096759:450580-450837 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References