Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1891257..1892032 Replicon chromosome
Accession NZ_CP084705
Organism Klebsiella pneumoniae strain KP1064WHY

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K8U55_RS09185 Protein ID WP_004150910.1
Coordinates 1891547..1892032 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K8U55_RS09180 Protein ID WP_004150912.1
Coordinates 1891257..1891550 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8U55_RS09160 (K8U55_09160) 1886465..1887067 - 603 WP_062954968.1 short chain dehydrogenase -
K8U55_RS09165 (K8U55_09165) 1887165..1888076 + 912 WP_004188550.1 LysR family transcriptional regulator -
K8U55_RS09170 (K8U55_09170) 1888077..1889225 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K8U55_RS09175 (K8U55_09175) 1889236..1890612 - 1377 WP_004217775.1 cystathionine beta-synthase -
K8U55_RS09180 (K8U55_09180) 1891257..1891550 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K8U55_RS09185 (K8U55_09185) 1891547..1892032 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K8U55_RS09190 (K8U55_09190) 1892736..1893329 + 594 WP_004188553.1 hypothetical protein -
K8U55_RS09195 (K8U55_09195) 1893426..1893642 + 217 Protein_1779 transposase -
K8U55_RS09200 (K8U55_09200) 1894248..1895120 + 873 WP_004188557.1 ParA family protein -
K8U55_RS09205 (K8U55_09205) 1895120..1895503 + 384 WP_004150906.1 hypothetical protein -
K8U55_RS09210 (K8U55_09210) 1895496..1896863 + 1368 WP_226522432.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1893426..1893578 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T219189 WP_004150910.1 NZ_CP084705:1891547-1892032 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T219189 NZ_CP116197:203854-203961 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT219189 WP_004150912.1 NZ_CP084705:1891257-1891550 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT219189 NZ_CP116197:c203805-203742 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References