Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 734963..735738 Replicon chromosome
Accession NZ_CP084830
Organism Klebsiella pneumoniae strain SB612

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A331A5C5
Locus tag LGM16_RS03655 Protein ID WP_019704987.1
Coordinates 735253..735738 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LGM16_RS03650 Protein ID WP_004150912.1
Coordinates 734963..735256 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM16_RS03630 (LGM16_03630) 730172..730774 - 603 WP_019704985.1 short chain dehydrogenase -
LGM16_RS03635 (LGM16_03635) 730872..731783 + 912 WP_017879796.1 LysR family transcriptional regulator -
LGM16_RS03640 (LGM16_03640) 731784..732932 - 1149 WP_019704986.1 PLP-dependent aspartate aminotransferase family protein -
LGM16_RS03645 (LGM16_03645) 732943..734319 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
LGM16_RS03650 (LGM16_03650) 734963..735256 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LGM16_RS03655 (LGM16_03655) 735253..735738 + 486 WP_019704987.1 GNAT family N-acetyltransferase Toxin
LGM16_RS03660 (LGM16_03660) 736442..737035 + 594 WP_004188553.1 hypothetical protein -
LGM16_RS03665 (LGM16_03665) 737132..737348 + 217 Protein_720 transposase -
LGM16_RS03675 (LGM16_03675) 738352..739065 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 737132..737284 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17572.56 Da        Isoelectric Point: 8.5111

>T219512 WP_019704987.1 NZ_CP084830:735253-735738 [Klebsiella pneumoniae]
MISAPEPLNAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T219512 NZ_CP116480:c4117040-4116762 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT219512 WP_004150912.1 NZ_CP084830:734963-735256 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT219512 NZ_CP116480:c4117303-4117043 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331A5C5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References