Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 804003..804778 Replicon chromosome
Accession NZ_CP058857
Organism Klebsiella pneumoniae strain STLE2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HZT19_RS04060 Protein ID WP_004150910.1
Coordinates 804293..804778 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HZT19_RS04055 Protein ID WP_004150912.1
Coordinates 804003..804296 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT19_RS04035 (HZT19_04035) 799211..799813 - 603 WP_002916735.1 short chain dehydrogenase -
HZT19_RS04040 (HZT19_04040) 799911..800822 + 912 WP_004188550.1 LysR family transcriptional regulator -
HZT19_RS04045 (HZT19_04045) 800823..801971 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HZT19_RS04050 (HZT19_04050) 801982..803358 - 1377 WP_004217775.1 cystathionine beta-synthase -
HZT19_RS04055 (HZT19_04055) 804003..804296 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HZT19_RS04060 (HZT19_04060) 804293..804778 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HZT19_RS04065 (HZT19_04065) 805482..806075 + 594 WP_004188553.1 hypothetical protein -
HZT19_RS04070 (HZT19_04070) 806172..806388 + 217 Protein_800 transposase -
HZT19_RS04075 (HZT19_04075) 806994..807866 + 873 WP_004188557.1 ParA family protein -
HZT19_RS04080 (HZT19_04080) 807866..808249 + 384 WP_004150906.1 hypothetical protein -
HZT19_RS04085 (HZT19_04085) 808242..809609 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 806172..806324 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T167762 WP_004150910.1 NZ_CP058857:804293-804778 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T167762 NZ_CP075452:c4675495-4675175 [Klebsiella pneumoniae]
ATGTACATTTCAGCTGTACCGGCCACGGTGCCGGTTTCGTCACGTCTGTCGCCCGTGCAGGTCTGGCAGCAACTTTTAAC
GTATCTGCTGGATCACCATTACGGCCTGACGCTTAACGACACACCATTCCATGACGACGCGGCCATCAAGGAGCATATCG
ATGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTTGTGGAACGTTATGAACTGGTACGCATCGACCGCAAAGGGTTT
AGCTGGCAGGAACAGACTCCGTTCCTGACCGCGACTGATATTCTCCGAGCCAGGCGAGCTACCGGATTAATAAATACATA
A

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT167762 WP_004150912.1 NZ_CP058857:804003-804296 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT167762 NZ_CP075452:c4675854-4675531 [Klebsiella pneumoniae]
ATGTCAATCACACCACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAATCGACTGCATTACCTGGCTGACAGGGCTGGATTGACGGGGAGTTTTAGCCCGATACACCTGCAGAAACTGGATCAGG
CTTTCCCGCTGTTTATAGAACAACTGGAAAATATGTTGCGCGTTGGCGAACTAAATCCACATCAGCAATATCATGTGACC
ATTCAACTTGCGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGTGGCTACGTTTACATTTCTATCTATCCAACCCA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References