Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 714870..715645 Replicon chromosome
Accession NZ_CP089986
Organism Klebsiella pneumoniae strain LZKP00001

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag LUA09_RS03540 Protein ID WP_004889762.1
Coordinates 715160..715645 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LUA09_RS03535 Protein ID WP_004150912.1
Coordinates 714870..715163 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUA09_RS03515 (LUA09_03515) 710079..710681 - 603 WP_004174410.1 short chain dehydrogenase -
LUA09_RS03520 (LUA09_03520) 710779..711690 + 912 WP_017879796.1 LysR family transcriptional regulator -
LUA09_RS03525 (LUA09_03525) 711691..712839 - 1149 WP_004889759.1 PLP-dependent aspartate aminotransferase family protein -
LUA09_RS03530 (LUA09_03530) 712850..714226 - 1377 WP_017879795.1 pyridoxal-phosphate dependent enzyme -
LUA09_RS03535 (LUA09_03535) 714870..715163 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LUA09_RS03540 (LUA09_03540) 715160..715645 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
LUA09_RS03545 (LUA09_03545) 716349..716941 + 593 Protein_697 hypothetical protein -
LUA09_RS03550 (LUA09_03550) 717038..717254 + 217 Protein_698 transposase -
LUA09_RS03560 (LUA09_03560) 717769..718482 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 717038..717190 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T229846 WP_004889762.1 NZ_CP089986:715160-715645 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T229846 NZ_CP124543:c5519720-5519358 [Halotia branconii CENA392]
ATGCAGAGTGATGAGGCGGTTATAATTCGGTTCTCTGATGAATTTGAGGAGGAACTTTACAGACTTTCCAAAAGATTTCG
CAATATTCGCTCTGATGTGCAGCCGATTATTGAACAATTACAACAAGGAAATATTATTGGAGATAGAATTGCAGGAATAG
GCGAGGAGTATATTGTTTATAAGGTGAGAGTTCGCAACAGTAATATTCAAAAAGGTAAAAGTGCTGGATATTGTTTAATT
TATCAAGTTGAGTCACCTACAACTATTTTGCTATTAACAATTTATTCTAAATCGGATCGAGAAGATATTGGTACAAATGA
AATTCGAGATATTGTAGCTGATTTTTATCATAAACAAGATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT229846 WP_004150912.1 NZ_CP089986:714870-715163 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT229846 NZ_CP124543:c5519967-5519710 [Halotia branconii CENA392]
ATGAAAGCGATTGAAGTTACTGGTAAAATTGATGCTCAAGGAAATTTAGTTTTGGATGAGCCAATTCTGGGAACGCCTTA
CCCTCATCAGGTGCGAGTAATTGTCTTAGTCCCAGAACAGGTAGAAGTAAAAGAGGTTGACCCTGATGATACACCTCTTG
AAGAGATTAAAGCCAGCTTAAGGAGAGCTTTGCAACAGGCAAAAGCCGGGCAAACTAGACCGATTAGTGAGCTATGGGAC
AGAATTGATGCAGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References