Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 768245..769020 Replicon chromosome
Accession NZ_CP047595
Organism Klebsiella pneumoniae strain Kpn 1693

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag GTK25_RS03845 Protein ID WP_004889762.1
Coordinates 768535..769020 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag GTK25_RS03840 Protein ID WP_004150912.1
Coordinates 768245..768538 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK25_RS03820 (763454) 763454..764056 - 603 WP_002916735.1 short chain dehydrogenase -
GTK25_RS03825 (764154) 764154..765065 + 912 WP_014906929.1 LysR family transcriptional regulator -
GTK25_RS03830 (765066) 765066..766214 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
GTK25_RS03835 (766225) 766225..767601 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
GTK25_RS03840 (768245) 768245..768538 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
GTK25_RS03845 (768535) 768535..769020 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
GTK25_RS03850 (769724) 769724..770317 + 594 WP_004188553.1 hypothetical protein -
GTK25_RS03855 (770414) 770414..770630 + 217 Protein_761 transposase -
GTK25_RS03865 (771308) 771308..772021 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 770414..770566 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T146843 WP_004889762.1 NZ_CP047595:768535-769020 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T146843 NZ_CP062811:2364902-2365189 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT146843 WP_004150912.1 NZ_CP047595:768245-768538 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT146843 NZ_CP062811:2364663-2364902 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References