Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 699202..699977 Replicon chromosome
Accession NZ_CP082796
Organism Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag K8B77_RS03495 Protein ID WP_032445441.1
Coordinates 699492..699977 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K8B77_RS03490 Protein ID WP_004150912.1
Coordinates 699202..699495 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B77_RS03470 (694410) 694410..695012 - 603 WP_020802400.1 short chain dehydrogenase -
K8B77_RS03475 (695110) 695110..696021 + 912 WP_004181308.1 LysR family transcriptional regulator -
K8B77_RS03480 (696022) 696022..697170 - 1149 WP_016947441.1 PLP-dependent aspartate aminotransferase family protein -
K8B77_RS03485 (697181) 697181..698557 - 1377 WP_072158501.1 cystathionine beta-synthase -
K8B77_RS03490 (699202) 699202..699495 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K8B77_RS03495 (699492) 699492..699977 + 486 WP_032445441.1 GNAT family N-acetyltransferase Toxin
K8B77_RS03500 (700681) 700681..701274 + 594 WP_032445442.1 hypothetical protein -
K8B77_RS03505 (701371) 701371..701587 + 217 Protein_688 transposase -
K8B77_RS03515 (702428) 702428..703141 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 701371..701523 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17580.59 Da        Isoelectric Point: 8.1558

>T215099 WP_032445441.1 NZ_CP082796:699492-699977 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGIGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T215099 NZ_CP110404:c3984747-3984436 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT215099 WP_004150912.1 NZ_CP082796:699202-699495 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT215099 NZ_CP110404:c3984439-3984020 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References