Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 732763..733538 Replicon chromosome
Accession NZ_CP113192
Organism Klebsiella pneumoniae strain SXC4-2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag OT483_RS03625 Protein ID WP_004150910.1
Coordinates 733053..733538 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag OT483_RS03620 Protein ID WP_004150912.1
Coordinates 732763..733056 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OT483_RS03600 (727977) 727977..728579 - 603 WP_002916735.1 short chain dehydrogenase -
OT483_RS03605 (728677) 728677..729588 + 912 WP_004188550.1 LysR family transcriptional regulator -
OT483_RS03610 (729589) 729589..730737 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
OT483_RS03615 (730748) 730748..732118 - 1371 WP_219716907.1 cystathionine beta-synthase -
OT483_RS03620 (732763) 732763..733056 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
OT483_RS03625 (733053) 733053..733538 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
OT483_RS03630 (734242) 734242..734835 + 594 WP_004188553.1 hypothetical protein -
OT483_RS03635 (734932) 734932..735148 + 217 Protein_713 transposase -
OT483_RS03640 (735754) 735754..736626 + 873 WP_004188557.1 ParA family protein -
OT483_RS03645 (736626) 736626..737009 + 384 WP_004150906.1 hypothetical protein -
OT483_RS03650 (737002) 737002..738369 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - rfaE 670186..799232 129046
flank IS/Tn - - 734932..735084 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T265542 WP_004150910.1 NZ_CP113192:733053-733538 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT265542 WP_004150912.1 NZ_CP113192:732763-733056 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References