Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 771479..772254 Replicon chromosome
Accession NZ_CP101835
Organism Klebsiella pneumoniae strain GN4549

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag NPH93_RS03900 Protein ID WP_004150910.1
Coordinates 771769..772254 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NPH93_RS03895 Protein ID WP_004150912.1
Coordinates 771479..771772 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPH93_RS03875 (NPH93_03875) 766687..767289 - 603 WP_002916735.1 short chain dehydrogenase -
NPH93_RS03880 (NPH93_03880) 767387..768298 + 912 WP_004188550.1 LysR family transcriptional regulator -
NPH93_RS03885 (NPH93_03885) 768299..769447 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
NPH93_RS03890 (NPH93_03890) 769458..770834 - 1377 WP_004217775.1 cystathionine beta-synthase -
NPH93_RS03895 (NPH93_03895) 771479..771772 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NPH93_RS03900 (NPH93_03900) 771769..772254 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
NPH93_RS03905 (NPH93_03905) 772958..773551 + 594 WP_004188553.1 hypothetical protein -
NPH93_RS03910 (NPH93_03910) 773648..773864 + 217 Protein_769 transposase -
NPH93_RS03915 (NPH93_03915) 774470..775342 + 873 WP_004188557.1 ParA family protein -
NPH93_RS03920 (NPH93_03920) 775342..775725 + 384 WP_004150906.1 hypothetical protein -
NPH93_RS03925 (NPH93_03925) 775718..777085 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 773648..773800 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T252745 WP_004150910.1 NZ_CP101835:771769-772254 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT252745 WP_004150912.1 NZ_CP101835:771479-771772 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References