Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737833..738608 Replicon chromosome
Accession NZ_CP061396
Organism Klebsiella pneumoniae strain NU-CRE193

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HCV83_RS03685 Protein ID WP_004150910.1
Coordinates 738123..738608 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HCV83_RS03680 Protein ID WP_004150912.1
Coordinates 737833..738126 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV83_RS03660 733041..733643 - 603 WP_002916735.1 short chain dehydrogenase -
HCV83_RS03665 733741..734652 + 912 WP_004188550.1 LysR family transcriptional regulator -
HCV83_RS03670 734653..735801 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HCV83_RS03675 735812..737188 - 1377 WP_004217775.1 cystathionine beta-synthase -
HCV83_RS03680 737833..738126 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HCV83_RS03685 738123..738608 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HCV83_RS03690 739312..739905 + 594 WP_004188553.1 hypothetical protein -
HCV83_RS03695 740002..740218 + 217 Protein_725 transposase -
HCV83_RS03700 740824..741696 + 873 WP_004188557.1 ParA family protein -
HCV83_RS03705 741696..742079 + 384 WP_004150906.1 hypothetical protein -
HCV83_RS03710 742072..743439 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 740002..740154 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T172592 WP_004150910.1 NZ_CP061396:738123-738608 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T172592 NZ_CP079802:c3504223-3504047 [Klebsiella variicola]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGCATCAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT172592 WP_004150912.1 NZ_CP061396:737833-738126 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT172592 NZ_CP079802:c3503999-3503592 [Klebsiella variicola]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References