Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 720676..721451 Replicon chromosome
Accession NZ_CP072200
Organism Klebsiella pneumoniae strain B5055

Toxin (Protein)


Gene name TacT2 Uniprot ID W9B2R2
Locus tag J6T75_RS03575 Protein ID WP_046042357.1
Coordinates 720966..721451 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag J6T75_RS03570 Protein ID WP_004150912.1
Coordinates 720676..720969 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6T75_RS03550 (J6T75_03540) 715884..716486 - 603 WP_004174410.1 short chain dehydrogenase -
J6T75_RS03555 (J6T75_03545) 716584..717495 + 912 WP_021314148.1 LysR family transcriptional regulator -
J6T75_RS03560 (J6T75_03550) 717496..718644 - 1149 WP_016531357.1 PLP-dependent aspartate aminotransferase family protein -
J6T75_RS03565 (J6T75_03555) 718655..720031 - 1377 WP_171819465.1 pyridoxal-phosphate dependent enzyme -
J6T75_RS03570 (J6T75_03560) 720676..720969 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
J6T75_RS03575 (J6T75_03565) 720966..721451 + 486 WP_046042357.1 GNAT family N-acetyltransferase Toxin
J6T75_RS03580 (J6T75_03570) 722155..722748 + 594 WP_004188553.1 hypothetical protein -
J6T75_RS03585 (J6T75_03575) 722845..723061 + 217 Protein_705 transposase -
J6T75_RS03595 (J6T75_03585) 723576..724289 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 722845..722997 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17611.64 Da        Isoelectric Point: 8.5144

>T197495 WP_046042357.1 NZ_CP072200:720966-721451 [Klebsiella pneumoniae]
MILAPESLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T197495 NZ_CP096219:489629-489970 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT197495 WP_004150912.1 NZ_CP072200:720676-720969 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT197495 NZ_CP096219:489399-489629 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGGAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9B2R2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References