Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4587114..4587889 Replicon chromosome
Accession NZ_CP065464
Organism Klebsiella pneumoniae strain Olaf

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I5M57_RS22665 Protein ID WP_004150910.1
Coordinates 4587114..4587599 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I5M57_RS22670 Protein ID WP_004150912.1
Coordinates 4587596..4587889 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M57_RS22640 4582283..4583650 - 1368 WP_004150905.1 replicative DNA helicase -
I5M57_RS22645 4583643..4584026 - 384 WP_004150906.1 hypothetical protein -
I5M57_RS22650 4584026..4584898 - 873 WP_004188557.1 ParA family protein -
I5M57_RS22655 4585504..4585720 - 217 Protein_4427 transposase -
I5M57_RS22660 4585817..4586410 - 594 WP_004188553.1 hypothetical protein -
I5M57_RS22665 4587114..4587599 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I5M57_RS22670 4587596..4587889 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I5M57_RS22675 4588534..4589910 + 1377 WP_194428446.1 cystathionine beta-synthase -
I5M57_RS22680 4589921..4591069 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I5M57_RS22685 4591070..4591981 - 912 WP_004188550.1 LysR family transcriptional regulator -
I5M57_RS22690 4592079..4592681 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4528788..4588140 59352
flank IS/Tn - - 4585568..4585720 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T182803 WP_004150910.1 NZ_CP065464:c4587599-4587114 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T182803 NZ_CP086592:2954845-2955009 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAACTATCGTTTATTGTACAACATATGAAACAATTCAGACGATCCTTGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAGAAGACAACAGAAATGACCGCTGTTTAACTTTGGCTA
GTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT182803 WP_004150912.1 NZ_CP065464:c4587889-4587596 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT182803 NZ_CP086592:c2955113-2954918 [Enterococcus faecalis]
TGTGCTGCAATAGAAACGAAAAGAGAGGTATGCTTCCAACATAGCTCTCTAATGTAGAACCGTTACTAACGGTGACAAAA
TTTTAAAGATTTTGAAATGACCGTCTAACTAGCCAAAGTTAAACAGCGGTCATTTCTGTTGTCTTCTTTAAGCAATTTCA
CAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References