Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 731780..732555 Replicon chromosome
Accession NZ_CP082290
Organism Klebsiella pneumoniae subsp. pneumoniae strain Kp6

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag K6T93_RS03665 Protein ID WP_009308645.1
Coordinates 732070..732555 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K6T93_RS03660 Protein ID WP_004150912.1
Coordinates 731780..732073 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T93_RS03640 (K6T93_03640) 726988..727590 - 603 WP_004174410.1 short chain dehydrogenase -
K6T93_RS03645 (K6T93_03645) 727688..728599 + 912 WP_057222543.1 LysR family transcriptional regulator -
K6T93_RS03650 (K6T93_03650) 728600..729748 - 1149 WP_016947441.1 PLP-dependent aspartate aminotransferase family protein -
K6T93_RS03655 (K6T93_03655) 729759..731135 - 1377 WP_072143321.1 cystathionine beta-synthase -
K6T93_RS03660 (K6T93_03660) 731780..732073 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K6T93_RS03665 (K6T93_03665) 732070..732555 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
K6T93_RS03670 (K6T93_03670) 733259..733852 + 594 WP_065519573.1 hypothetical protein -
K6T93_RS03675 (K6T93_03675) 733949..734165 + 217 Protein_723 transposase -
K6T93_RS03685 (K6T93_03685) 734843..735556 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 733949..734101 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T214511 WP_009308645.1 NZ_CP082290:732070-732555 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T214511 NZ_CP110118:78290-78688 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCTG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT214511 WP_004150912.1 NZ_CP082290:731780-732073 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT214511 NZ_CP110118:78063-78290 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTATCGGACGAACCCGACTCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAACGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References