Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4937243..4938018 Replicon chromosome
Accession NZ_CP020837
Organism Klebsiella pneumoniae strain BK13043

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag B9G35_RS24820 Protein ID WP_004150910.1
Coordinates 4937243..4937728 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag B9G35_RS24825 Protein ID WP_004150912.1
Coordinates 4937725..4938018 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9G35_RS24790 4932412..4933779 - 1368 WP_004150905.1 replicative DNA helicase -
B9G35_RS24795 4933772..4934155 - 384 WP_004150906.1 hypothetical protein -
B9G35_RS24800 4934155..4935027 - 873 WP_004188557.1 ParA family protein -
B9G35_RS24810 4935633..4935849 - 217 Protein_4727 transposase -
B9G35_RS24815 4935946..4936539 - 594 WP_004188553.1 hypothetical protein -
B9G35_RS24820 4937243..4937728 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
B9G35_RS24825 4937725..4938018 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
B9G35_RS24830 4938663..4940039 + 1377 WP_004217775.1 cystathionine beta-synthase -
B9G35_RS24835 4940050..4941198 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
B9G35_RS24840 4941199..4942110 - 912 WP_004188550.1 LysR family transcriptional regulator -
B9G35_RS24845 4942208..4942810 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4880222..4938269 58047
flank IS/Tn - - 4935697..4935849 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T75504 WP_004150910.1 NZ_CP020837:c4937728-4937243 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T75504 NZ_CP020837:c4937728-4937243 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT75504 WP_004150912.1 NZ_CP020837:c4938018-4937725 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT75504 NZ_CP020837:c4938018-4937725 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References