Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1272215..1272990 Replicon chromosome
Accession NZ_CP047160
Organism Klebsiella pneumoniae strain KP19-2029

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag GSU00_RS06215 Protein ID WP_004150910.1
Coordinates 1272505..1272990 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag GSU00_RS06210 Protein ID WP_004150912.1
Coordinates 1272215..1272508 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSU00_RS06190 1267423..1268025 - 603 WP_062954968.1 short chain dehydrogenase -
GSU00_RS06195 1268123..1269034 + 912 WP_004188550.1 LysR family transcriptional regulator -
GSU00_RS06200 1269035..1270183 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
GSU00_RS06205 1270194..1271570 - 1377 WP_004217775.1 cystathionine beta-synthase -
GSU00_RS06210 1272215..1272508 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
GSU00_RS06215 1272505..1272990 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
GSU00_RS06220 1273694..1274287 + 594 WP_004188553.1 hypothetical protein -
GSU00_RS06225 1274384..1274600 + 217 Protein_1210 transposase -
GSU00_RS06240 1275206..1276078 + 873 WP_004188557.1 ParA family protein -
GSU00_RS06245 1276078..1276461 + 384 WP_004150906.1 hypothetical protein -
GSU00_RS06250 1276454..1277821 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1274384..1274536 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T145504 WP_004150910.1 NZ_CP047160:1272505-1272990 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T145504 NZ_CP062399:c1958682-1958530 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGCCAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT145504 WP_004150912.1 NZ_CP047160:1272215-1272508 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT145504 NZ_CP062399:c1959306-1958707 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References