Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742899..743674 Replicon chromosome
Accession NZ_CP087651
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01653

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LL272_RS03670 Protein ID WP_004150910.1
Coordinates 743189..743674 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LL272_RS03665 Protein ID WP_004150912.1
Coordinates 742899..743192 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LL272_RS03645 (LL272_03645) 738107..738709 - 603 WP_062954968.1 short chain dehydrogenase -
LL272_RS03650 (LL272_03650) 738807..739718 + 912 WP_004188550.1 LysR family transcriptional regulator -
LL272_RS03655 (LL272_03655) 739719..740867 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LL272_RS03660 (LL272_03660) 740878..742254 - 1377 WP_004217775.1 cystathionine beta-synthase -
LL272_RS03665 (LL272_03665) 742899..743192 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LL272_RS03670 (LL272_03670) 743189..743674 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LL272_RS03675 (LL272_03675) 744378..744971 + 594 WP_004188553.1 hypothetical protein -
LL272_RS03680 (LL272_03680) 745068..745284 + 217 Protein_724 transposase -
LL272_RS03685 (LL272_03685) 745890..746762 + 873 WP_004188557.1 ParA family protein -
LL272_RS03690 (LL272_03690) 746762..747145 + 384 WP_004150906.1 hypothetical protein -
LL272_RS03695 (LL272_03695) 747138..748505 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 745068..745220 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T225834 WP_004150910.1 NZ_CP087651:743189-743674 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T225834 NZ_CP121376:c16504-16208 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTGCCTGAATTTGATGAATGGCTAGATGGTCTTAAAGACAATATGACTCGAATCCGCTTGAA
TCGTCGATTAGATAAGGTCCAACGTGGTAATTGGGGTGACATTAAACCACTCCAAGATGGTGTCTGGGAAATGAGAGAGT
TCTTTGGTGCTGGTTGGCGGATGTACTACATCCAACATGGTGACGTTGTGATCGTGATGCTTGGTGGTGGTGAAAAATCT
ACACAGAAAGATGACATCAAGAAAGCGGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT225834 WP_004150912.1 NZ_CP087651:742899-743192 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT225834 NZ_CP121376:c16206-15916 [Acinetobacter baumannii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGATATTGTAATGTACCTCAA
TATGGTTCTTGAAGAAAATGATCCTGCTGAATTAGCGCACGCACTTGGTGTAATTGCTAAAGCAAGAGGTATGACCCAAA
TTGCGAAAGAAGCAGGTATTGGACGAGAAGCCCTTTACAAAGCACTTCGTCAAGACTCAACGCCTCGTTTTGATACGATC
AACCGTGTTGTGAATGCTTTAGGTTTAAAATTGACAGTTCAACATACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References