Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739499..740274 Replicon chromosome
Accession NZ_CP087151
Organism Klebsiella pneumoniae strain KP14

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LOX93_RS03695 Protein ID WP_004150910.1
Coordinates 739789..740274 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LOX93_RS03690 Protein ID WP_004150912.1
Coordinates 739499..739792 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX93_RS03670 (LOX93_03670) 734707..735309 - 603 WP_062954968.1 short chain dehydrogenase -
LOX93_RS03675 (LOX93_03675) 735407..736318 + 912 WP_004188550.1 LysR family transcriptional regulator -
LOX93_RS03680 (LOX93_03680) 736319..737467 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LOX93_RS03685 (LOX93_03685) 737478..738854 - 1377 WP_004217775.1 cystathionine beta-synthase -
LOX93_RS03690 (LOX93_03690) 739499..739792 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LOX93_RS03695 (LOX93_03695) 739789..740274 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LOX93_RS03700 (LOX93_03700) 740978..741571 + 594 WP_004188553.1 hypothetical protein -
LOX93_RS03705 (LOX93_03705) 741668..741884 + 217 Protein_728 transposase -
LOX93_RS03710 (LOX93_03710) 742490..743362 + 873 WP_004188557.1 ParA family protein -
LOX93_RS03715 (LOX93_03715) 743362..743745 + 384 WP_004150906.1 hypothetical protein -
LOX93_RS03720 (LOX93_03720) 743738..745105 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 741668..741820 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T224917 WP_004150910.1 NZ_CP087151:739789-740274 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T224917 NZ_CP120622:488754-489104 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT224917 WP_004150912.1 NZ_CP087151:739499-739792 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT224917 NZ_CP120622:488468-488749 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References