Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741248..742023 Replicon chromosome
Accession NZ_CP071086
Organism Klebsiella pneumoniae strain 3347689I

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag JY394_RS03680 Protein ID WP_004150910.1
Coordinates 741538..742023 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag JY394_RS03675 Protein ID WP_004150912.1
Coordinates 741248..741541 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY394_RS03655 (JY394_03655) 736456..737058 - 603 WP_002916735.1 short chain dehydrogenase -
JY394_RS03660 (JY394_03660) 737156..738067 + 912 WP_004188550.1 LysR family transcriptional regulator -
JY394_RS03665 (JY394_03665) 738068..739216 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
JY394_RS03670 (JY394_03670) 739227..740603 - 1377 WP_004217775.1 cystathionine beta-synthase -
JY394_RS03675 (JY394_03675) 741248..741541 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
JY394_RS03680 (JY394_03680) 741538..742023 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
JY394_RS03685 (JY394_03685) 742727..743320 + 594 WP_004188553.1 hypothetical protein -
JY394_RS03690 (JY394_03690) 743417..743633 + 217 Protein_724 transposase -
JY394_RS03695 (JY394_03695) 744239..745111 + 873 WP_004188557.1 ParA family protein -
JY394_RS03700 (JY394_03700) 745111..745494 + 384 WP_004150906.1 hypothetical protein -
JY394_RS03705 (JY394_03705) 745487..746854 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743417..743569 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T195259 WP_004150910.1 NZ_CP071086:741538-742023 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T195259 NZ_CP094460:c1181078-1180659 [Burkholderia pyrrocinia]
ATGATCCTTGTTGATACGAACGTCATTTTCGAACCGCTGCGACGCGAGCCGAGCGCGGCCGTGATCGAGTGGCTCGATGC
CCAGAACGTCGAGACGCTGTTTCTTGCTGCAATCAGTCTCGCGGAGATGCGATTCGGCGTGGCTGTATTGCCGGAAGGGC
GCAGGCGCGAGTGGCTGCATCAAAGCATCGAACGACGTGTCGTGCCGCTGTTTCGAGGCCGGATCCTGCCGTTCGATGAT
GCAGCGAGCAAAGCGTATGCGAGCCTTCGGGCGCGGGCCCGTGCCGCAGGTAGCGCGATCGCGCCTGTCGATTGTTTTAT
CGCCGCCACGGCCGAGGCGAACGGCCTGATCGTGGCCACGCGCGATGTCGCGCCGTTCGAGGCGGTGGGGCTTCGCGTGA
TCGATCCGTGGGCGCGGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT195259 WP_004150912.1 NZ_CP071086:741248-741541 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT195259 NZ_CP094460:c1181314-1181075 [Burkholderia pyrrocinia]
ATGCCGGTGATCACCGTTCGAAATTTGCCTGATGAAGTGCATCGCGCACTTCGGATTCGCGCGGCCCAGCACGGACGCAG
CACCGAAGCCGAGGTACGTGACATTCTCGAGCAGGCCGTCCTGCCAGGCGGGCGGCTCAAGCTGGGAACGTTGCTGGCGG
AAATCGGGCGGGAGGCTGGTGGAGTCGATTTCGACGTCCAGCGCGACAAGACGTCAACCGATCCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References