Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2996178..2996953 Replicon chromosome
Accession NZ_CP095240
Organism Klebsiella pneumoniae strain 21080237

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MU300_RS14820 Protein ID WP_004150910.1
Coordinates 2996468..2996953 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MU300_RS14815 Protein ID WP_004150912.1
Coordinates 2996178..2996471 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU300_RS14795 (MU300_14790) 2991386..2991988 - 603 WP_062954968.1 short chain dehydrogenase -
MU300_RS14800 (MU300_14795) 2992086..2992997 + 912 WP_004188550.1 LysR family transcriptional regulator -
MU300_RS14805 (MU300_14800) 2992998..2994146 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MU300_RS14810 (MU300_14805) 2994157..2995533 - 1377 WP_004217775.1 cystathionine beta-synthase -
MU300_RS14815 (MU300_14810) 2996178..2996471 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MU300_RS14820 (MU300_14815) 2996468..2996953 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MU300_RS14825 (MU300_14820) 2997657..2998250 + 594 WP_004188553.1 hypothetical protein -
MU300_RS14830 (MU300_14825) 2998347..2998563 + 217 Protein_2915 transposase -
MU300_RS14835 (MU300_14830) 2999169..3000041 + 873 WP_004188557.1 ParA family protein -
MU300_RS14840 (MU300_14835) 3000041..3000424 + 384 WP_004150906.1 hypothetical protein -
MU300_RS14845 (MU300_14840) 3000417..3001784 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2998347..2998499 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T241738 WP_004150910.1 NZ_CP095240:2996468-2996953 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T241738 NZ_OU015323:4135218-4135616 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT241738 WP_004150912.1 NZ_CP095240:2996178-2996471 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT241738 NZ_OU015323:4134991-4135218 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References