Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 747139..747914 Replicon chromosome
Accession NZ_CP071464
Organism Klebsiella pneumoniae strain C2244

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag J1C69_RS03735 Protein ID WP_004150910.1
Coordinates 747429..747914 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag J1C69_RS03730 Protein ID WP_004150912.1
Coordinates 747139..747432 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1C69_RS03710 742347..742949 - 603 WP_062954968.1 short chain dehydrogenase -
J1C69_RS03715 743047..743958 + 912 WP_004188550.1 LysR family transcriptional regulator -
J1C69_RS03720 743959..745107 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
J1C69_RS03725 745118..746494 - 1377 WP_004217775.1 cystathionine beta-synthase -
J1C69_RS03730 747139..747432 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
J1C69_RS03735 747429..747914 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
J1C69_RS03740 748618..749211 + 594 WP_004188553.1 hypothetical protein -
J1C69_RS03745 749308..749524 + 217 Protein_736 transposase -
J1C69_RS03750 750130..751002 + 873 WP_004188557.1 ParA family protein -
J1C69_RS03755 751002..751385 + 384 WP_004150906.1 hypothetical protein -
J1C69_RS03760 751378..752745 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 749308..749460 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T196444 WP_004150910.1 NZ_CP071464:747429-747914 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T196444 NZ_CP095125:c3992799-3992512 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT196444 WP_004150912.1 NZ_CP071464:747139-747432 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT196444 NZ_CP095125:c3992525-3992223 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References