Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739264..740039 Replicon chromosome
Accession NZ_CP071261
Organism Klebsiella pneumoniae strain MEI004

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag J0G27_RS03670 Protein ID WP_009308645.1
Coordinates 739554..740039 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag J0G27_RS03665 Protein ID WP_004150912.1
Coordinates 739264..739557 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G27_RS03645 (734473) 734473..735075 - 603 WP_004174410.1 short chain dehydrogenase -
J0G27_RS03650 (735173) 735173..736084 + 912 WP_004181308.1 LysR family transcriptional regulator -
J0G27_RS03655 (736085) 736085..737233 - 1149 WP_250354829.1 PLP-dependent aspartate aminotransferase family protein -
J0G27_RS03660 (737244) 737244..738620 - 1377 WP_250354830.1 cystathionine beta-synthase -
J0G27_RS03665 (739264) 739264..739557 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
J0G27_RS03670 (739554) 739554..740039 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
J0G27_RS03675 (740743) 740743..741336 + 594 WP_004188553.1 hypothetical protein -
J0G27_RS03680 (741433) 741433..741649 + 217 Protein_721 transposase -
J0G27_RS03690 (742164) 742164..742877 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 741433..741585 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T196058 WP_009308645.1 NZ_CP071261:739554-740039 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T196058 NZ_CP095020:c1389013-1388720 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT196058 WP_004150912.1 NZ_CP071261:739264-739557 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT196058 NZ_CP095020:c1389279-1389010 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References