Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1749084..1749859 Replicon chromosome
Accession NZ_CP069940
Organism Klebsiella pneumoniae strain FDAARGOS_1312

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag I6K42_RS08265 Protein ID WP_004889762.1
Coordinates 1749084..1749569 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I6K42_RS08270 Protein ID WP_004150912.1
Coordinates 1749566..1749859 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K42_RS08245 1746083..1746796 + 714 WP_002916694.1 DUF554 domain-containing protein -
I6K42_RS08255 1747474..1747690 - 217 Protein_1607 transposase -
I6K42_RS08260 1747787..1748380 - 594 WP_004188553.1 hypothetical protein -
I6K42_RS08265 1749084..1749569 - 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
I6K42_RS08270 1749566..1749859 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I6K42_RS08275 1750503..1751879 + 1377 WP_161799263.1 pyridoxal-phosphate dependent enzyme -
I6K42_RS08280 1751890..1753038 + 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
I6K42_RS08285 1753039..1753950 - 912 WP_014906929.1 LysR family transcriptional regulator -
I6K42_RS08290 1754048..1754650 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1747538..1747690 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T192197 WP_004889762.1 NZ_CP069940:c1749569-1749084 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T192197 NZ_CP092179:c1840831-1840490 [Macrococcus brunensis]
ATGAGGCGCGGCGAAGTCTTCCTGGCTGATCTGTCCCCTGTCAAAGGCTCGGAACAAGGCGGTAAGCGTCCTGTTGTCAT
TATTCAGAACGATGTCGGGAATAAGTACAGTCCGACTGTTATCGTGGCTGCTATCACAGCGAAAATCAATAAAGCACGTA
TTCCGACCCATGTAGAGATTGGTAAAACCAATCATCATCTGGATAAGGATTCAGTGATACTGCTTGAACAGATCAGAACG
ATTGATAAAAATCGGTTGATTGAGAAGCTGACTGTTCTCTCTGCGGAAAAAATGCATGAAGTGAACCAGGCGCTTGAAAT
CAGTCTCGGACTTACTCAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT192197 WP_004150912.1 NZ_CP069940:c1749859-1749566 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT192197 NZ_CP092179:c1840975-1840832 [Macrococcus brunensis]
ATGAAGAAATTTGATCAATTATTAGCTGAAGGCTATGAATCTATGGCTGAACTTAATTTAATCATTTCTTATGAGCAATT
CGATCTTGAAAGTGAAGTATGTGAAAAGAGCGAAATGTATTTCTTATCTGATGATGGTGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References