Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 738893..739668 Replicon chromosome
Accession NZ_CP076518
Organism Klebsiella pneumoniae strain K186

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KNW01_RS03685 Protein ID WP_004150910.1
Coordinates 739183..739668 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KNW01_RS03680 Protein ID WP_004150912.1
Coordinates 738893..739186 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNW01_RS03660 (KNW01_03660) 734101..734703 - 603 WP_002916735.1 short chain dehydrogenase -
KNW01_RS03665 (KNW01_03665) 734801..735712 + 912 WP_004188550.1 LysR family transcriptional regulator -
KNW01_RS03670 (KNW01_03670) 735713..736861 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KNW01_RS03675 (KNW01_03675) 736872..738248 - 1377 WP_004217775.1 cystathionine beta-synthase -
KNW01_RS03680 (KNW01_03680) 738893..739186 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KNW01_RS03685 (KNW01_03685) 739183..739668 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KNW01_RS03690 (KNW01_03690) 740372..740965 + 594 WP_004188553.1 hypothetical protein -
KNW01_RS03695 (KNW01_03695) 741062..741278 + 217 Protein_727 transposase -
KNW01_RS03700 (KNW01_03700) 741884..742756 + 873 WP_004188557.1 ParA family protein -
KNW01_RS03705 (KNW01_03705) 742756..743139 + 384 WP_004150906.1 hypothetical protein -
KNW01_RS03710 (KNW01_03710) 743132..744499 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 741062..741214 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T205891 WP_004150910.1 NZ_CP076518:739183-739668 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T205891 NZ_CP101922:c590181-589717 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT205891 WP_004150912.1 NZ_CP076518:738893-739186 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT205891 NZ_CP101922:c590516-590181 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References