Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739850..740625 Replicon chromosome
Accession NZ_AP024766
Organism Klebsiella pneumoniae strain TUM12131

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K6J85_RS03695 Protein ID WP_004150910.1
Coordinates 740140..740625 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K6J85_RS03690 Protein ID WP_004150912.1
Coordinates 739850..740143 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J85_RS03670 (TUM12131_07130) 735058..735660 - 603 WP_002916735.1 short chain dehydrogenase -
K6J85_RS03675 (TUM12131_07140) 735758..736669 + 912 WP_004188550.1 LysR family transcriptional regulator -
K6J85_RS03680 (TUM12131_07150) 736670..737818 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K6J85_RS03685 (TUM12131_07160) 737829..739205 - 1377 WP_004217775.1 cystathionine beta-synthase -
K6J85_RS03690 (TUM12131_07170) 739850..740143 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K6J85_RS03695 (TUM12131_07180) 740140..740625 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K6J85_RS03700 (TUM12131_07200) 741329..741922 + 594 WP_004188553.1 hypothetical protein -
K6J85_RS03705 742019..742235 + 217 Protein_729 transposase -
K6J85_RS03710 (TUM12131_07210) 742841..743713 + 873 WP_004188557.1 ParA family protein -
K6J85_RS03715 (TUM12131_07220) 743713..744096 + 384 WP_004150906.1 hypothetical protein -
K6J85_RS03720 (TUM12131_07230) 744089..745456 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 742019..742171 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T39524 WP_004150910.1 NZ_AP024766:740140-740625 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T39524 NZ_AP024766:740140-740625 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT39524 WP_004150912.1 NZ_AP024766:739850-740143 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT39524 NZ_AP024766:739850-740143 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References