Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 731853..732628 Replicon chromosome
Accession NZ_CP089021
Organism Klebsiella pneumoniae strain 53370

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag LRZ76_RS03620 Protein ID WP_004889762.1
Coordinates 732143..732628 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LRZ76_RS03615 Protein ID WP_004150912.1
Coordinates 731853..732146 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ76_RS03595 (727062) 727062..727664 - 603 WP_002916735.1 short chain dehydrogenase -
LRZ76_RS03600 (727762) 727762..728673 + 912 WP_014906929.1 LysR family transcriptional regulator -
LRZ76_RS03605 (728674) 728674..729822 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
LRZ76_RS03610 (729833) 729833..731209 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
LRZ76_RS03615 (731853) 731853..732146 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LRZ76_RS03620 (732143) 732143..732628 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
LRZ76_RS03625 (733332) 733332..733925 + 594 WP_004188553.1 hypothetical protein -
LRZ76_RS03630 (734022) 734022..734238 + 217 Protein_714 transposase -
LRZ76_RS03640 (734916) 734916..735629 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 734022..734174 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T227386 WP_004889762.1 NZ_CP089021:732143-732628 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T227386 NZ_CP122859:c4053373-4052996 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCTGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGTCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT227386 WP_004150912.1 NZ_CP089021:731853-732146 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT227386 NZ_CP122859:c4053831-4053463 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGAGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References