Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 737795..738570 Replicon chromosome
Accession NZ_CP055005
Organism Klebsiella pneumoniae strain STIN_77

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HUZ53_RS03685 Protein ID WP_004150910.1
Coordinates 738085..738570 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HUZ53_RS03680 Protein ID WP_004150912.1
Coordinates 737795..738088 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ53_RS03660 (HUZ53_03655) 733003..733605 - 603 WP_002916735.1 short chain dehydrogenase -
HUZ53_RS03665 (HUZ53_03660) 733703..734614 + 912 WP_004188550.1 LysR family transcriptional regulator -
HUZ53_RS03670 (HUZ53_03665) 734615..735763 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HUZ53_RS03675 (HUZ53_03670) 735774..737150 - 1377 WP_004217775.1 cystathionine beta-synthase -
HUZ53_RS03680 (HUZ53_03675) 737795..738088 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HUZ53_RS03685 (HUZ53_03680) 738085..738570 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HUZ53_RS03690 (HUZ53_03685) 739274..739867 + 594 WP_004188553.1 hypothetical protein -
HUZ53_RS03695 (HUZ53_03690) 739964..740180 + 217 Protein_726 transposase -
HUZ53_RS03700 (HUZ53_03695) 740786..741658 + 873 WP_004188557.1 ParA family protein -
HUZ53_RS03705 (HUZ53_03700) 741658..742041 + 384 WP_004150906.1 hypothetical protein -
HUZ53_RS03710 (HUZ53_03705) 742034..743401 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 739964..740116 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T160936 WP_004150910.1 NZ_CP055005:738085-738570 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T160936 NZ_CP070970:4177991-4178374 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT160936 WP_004150912.1 NZ_CP055005:737795-738088 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT160936 NZ_CP070970:4177749-4177991 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References