Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4398616..4399391 Replicon chromosome
Accession NZ_CP095776
Organism Klebsiella pneumoniae strain bo714

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MX575_RS21700 Protein ID WP_004150910.1
Coordinates 4398616..4399101 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MX575_RS21705 Protein ID WP_004150912.1
Coordinates 4399098..4399391 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MX575_RS21675 (MX575_21675) 4393785..4395152 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
MX575_RS21680 (MX575_21680) 4395145..4395528 - 384 WP_004150906.1 hypothetical protein -
MX575_RS21685 (MX575_21685) 4395528..4396400 - 873 WP_004188557.1 ParA family protein -
MX575_RS21690 (MX575_21690) 4397006..4397222 - 217 Protein_4263 transposase -
MX575_RS21695 (MX575_21695) 4397319..4397912 - 594 WP_004188553.1 hypothetical protein -
MX575_RS21700 (MX575_21700) 4398616..4399101 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MX575_RS21705 (MX575_21705) 4399098..4399391 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MX575_RS21710 (MX575_21710) 4400036..4401412 + 1377 WP_004217775.1 cystathionine beta-synthase -
MX575_RS21715 (MX575_21715) 4401423..4402571 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MX575_RS21720 (MX575_21720) 4402572..4403483 - 912 WP_004188550.1 LysR family transcriptional regulator -
MX575_RS21725 (MX575_21725) 4403581..4404183 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4341595..4399642 58047
flank IS/Tn - - 4397070..4397222 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T242546 WP_004150910.1 NZ_CP095776:c4399101-4398616 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T242546 NZ_OW848785:c234803-233481 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT242546 WP_004150912.1 NZ_CP095776:c4399391-4399098 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT242546 NZ_OW848785:c235069-234803 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References