Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739817..740592 Replicon chromosome
Accession NZ_CP110957
Organism Klebsiella pneumoniae subsp. pneumoniae strain LC-395/19

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag NPY03_RS03695 Protein ID WP_004150910.1
Coordinates 740107..740592 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NPY03_RS03690 Protein ID WP_004150912.1
Coordinates 739817..740110 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPY03_RS03670 (NPY03_03670) 735025..735627 - 603 WP_002916735.1 short chain dehydrogenase -
NPY03_RS03675 (NPY03_03675) 735725..736636 + 912 WP_004188550.1 LysR family transcriptional regulator -
NPY03_RS03680 (NPY03_03680) 736637..737785 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
NPY03_RS03685 (NPY03_03685) 737796..739172 - 1377 WP_004217775.1 cystathionine beta-synthase -
NPY03_RS03690 (NPY03_03690) 739817..740110 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NPY03_RS03695 (NPY03_03695) 740107..740592 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
NPY03_RS03700 (NPY03_03700) 741296..741889 + 594 WP_004188553.1 hypothetical protein -
NPY03_RS03705 (NPY03_03705) 741986..742202 + 217 Protein_729 transposase -
NPY03_RS03710 (NPY03_03710) 742808..743680 + 873 WP_004188557.1 ParA family protein -
NPY03_RS03715 (NPY03_03715) 743680..744063 + 384 WP_004150906.1 hypothetical protein -
NPY03_RS03720 (NPY03_03720) 744056..745423 + 1368 WP_265892302.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 741986..742138 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T264592 WP_004150910.1 NZ_CP110957:740107-740592 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT264592 WP_004150912.1 NZ_CP110957:739817-740110 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References