Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4620054..4620829 Replicon chromosome
Accession NZ_CP008827
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KPNIH1_RS23330 Protein ID WP_004150910.1
Coordinates 4620054..4620539 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KPNIH1_RS23335 Protein ID WP_004150912.1
Coordinates 4620536..4620829 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH1_RS23305 4615223..4616590 - 1368 WP_004150905.1 replicative DNA helicase -
KPNIH1_RS23310 4616583..4616966 - 384 WP_004150906.1 hypothetical protein -
KPNIH1_RS23315 4616966..4617838 - 873 WP_004188557.1 ParA family protein -
KPNIH1_RS23320 4618444..4618660 - 217 Protein_4448 transposase -
KPNIH1_RS23325 4618757..4619350 - 594 WP_004188553.1 hypothetical protein -
KPNIH1_RS23330 4620054..4620539 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KPNIH1_RS23335 4620536..4620829 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KPNIH1_RS23340 4621474..4622850 + 1377 WP_004217775.1 cystathionine beta-synthase -
KPNIH1_RS23345 4622861..4624009 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KPNIH1_RS23350 4624010..4624921 - 912 WP_004188550.1 LysR family transcriptional regulator -
KPNIH1_RS23355 4625019..4625621 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4561833..4621080 59247
flank IS/Tn - - 4618508..4618660 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T47660 WP_004150910.1 NZ_CP008827:c4620539-4620054 [Klebsiella pneumoniae subsp. pneumoniae KPNIH1]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T47660 NZ_CP008827:c4620539-4620054 [Klebsiella pneumoniae subsp. pneumoniae KPNIH1]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT47660 WP_004150912.1 NZ_CP008827:c4620829-4620536 [Klebsiella pneumoniae subsp. pneumoniae KPNIH1]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT47660 NZ_CP008827:c4620829-4620536 [Klebsiella pneumoniae subsp. pneumoniae KPNIH1]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References