Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 750326..751101 Replicon chromosome
Accession NZ_CP069046
Organism Klebsiella pneumoniae strain 33Kpn22

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag IFY68_RS03730 Protein ID WP_004150910.1
Coordinates 750616..751101 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag IFY68_RS03725 Protein ID WP_004150912.1
Coordinates 750326..750619 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFY68_RS03705 745534..746136 - 603 WP_002916735.1 short chain dehydrogenase -
IFY68_RS03710 746234..747145 + 912 WP_004188550.1 LysR family transcriptional regulator -
IFY68_RS03715 747146..748294 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
IFY68_RS03720 748305..749681 - 1377 WP_004217775.1 cystathionine beta-synthase -
IFY68_RS03725 750326..750619 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
IFY68_RS03730 750616..751101 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
IFY68_RS03735 751805..752398 + 594 WP_004188553.1 hypothetical protein -
IFY68_RS03740 752495..752711 + 217 Protein_735 transposase -
IFY68_RS03745 753317..754189 + 873 WP_004188557.1 ParA family protein -
IFY68_RS03750 754189..754572 + 384 WP_004150906.1 hypothetical protein -
IFY68_RS03755 754565..755932 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 752495..752647 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T188837 WP_004150910.1 NZ_CP069046:750616-751101 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T188837 NZ_CP090582:40093-40383 [Methylobacterium radiotolerans]
ATGAGTGACTACACCCTCACGCGGAAAGCCGAGGCGGACCTGTTCGACATCGCCGTCCACGGCTACCGGCAGTTCGGTGT
CCACCAAGCCGAAGCCTACGCCGCCGGTCTGGAGCAGGCCTTTCAGCTCCTCGCTGACAATCCTGGAATGGGCCGCAAGG
CCGACGGGATCCGCCCGGGCGTGCACCGGCATGAGCATGCCTCCCACGTCATCCTGTACGAGGTGGTGACTGGTGGCGTC
CTGATCCTGGCCATCATCCACGGCCGCAGCGTGAGGCGGCTCGCACTGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT188837 WP_004150912.1 NZ_CP069046:750326-750619 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT188837 NZ_CP090582:39831-40100 [Methylobacterium radiotolerans]
ATGGCGACCATGACCATCTCTCTGCCCGACCCGATGAAGGAGTGGATCGAGGCGCAGATCAGGCAGGGCGATTACGCCAG
CACGAGCGACTATGTCCGCGATCTCGTCCGCCGGGACCGGGAGCGGCGTGCCCATCCCGAACTGACGCTCGAGGATCTGC
GCCGGATCGTCGATGACGCTCGCGCCAGCGGACCGAGCCGGCGCAAGGTCCCTGAGATCCTCGCCCGGGCCAAGAAGCAC
GCGCAGGCCGCTCAGCCGCTGGATGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References