Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 730606..731381 Replicon chromosome
Accession NZ_CP096230
Organism Klebsiella pneumoniae subsp. pneumoniae strain H39

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag M0538_RS03615 Protein ID WP_004889762.1
Coordinates 730896..731381 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag M0538_RS03610 Protein ID WP_004150912.1
Coordinates 730606..730899 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0538_RS03590 (725815) 725815..726417 - 603 WP_002916735.1 short chain dehydrogenase -
M0538_RS03595 (726515) 726515..727426 + 912 WP_014906929.1 LysR family transcriptional regulator -
M0538_RS03600 (727427) 727427..728575 - 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
M0538_RS03605 (728586) 728586..729962 - 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
M0538_RS03610 (730606) 730606..730899 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
M0538_RS03615 (730896) 730896..731381 + 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
M0538_RS03620 (732085) 732085..732678 + 594 WP_004188553.1 hypothetical protein -
M0538_RS03625 (732775) 732775..732991 + 217 Protein_713 transposase -
M0538_RS03635 (733669) 733669..734382 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 732775..732927 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(41-143)

Antitoxin

(3-95)


Sequences


Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T242895 WP_004889762.1 NZ_CP096230:730896-731381 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T242895 NZ_OW967976:78500-78898 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT242895 WP_004150912.1 NZ_CP096230:730606-730899 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT242895 NZ_OW967976:78273-78500 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A447W563


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References