Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4461600..4462375 Replicon chromosome
Accession NZ_CP073791
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2010_71621

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KFO61_RS21790 Protein ID WP_004150910.1
Coordinates 4461600..4462085 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KFO61_RS21795 Protein ID WP_004150912.1
Coordinates 4462082..4462375 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO61_RS21765 (KFO61_21765) 4456769..4458136 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
KFO61_RS21770 (KFO61_21770) 4458129..4458512 - 384 WP_004150906.1 hypothetical protein -
KFO61_RS21775 (KFO61_21775) 4458512..4459384 - 873 WP_004188557.1 ParA family protein -
KFO61_RS21780 (KFO61_21780) 4459990..4460206 - 217 Protein_4260 transposase -
KFO61_RS21785 (KFO61_21785) 4460303..4460896 - 594 WP_004188553.1 hypothetical protein -
KFO61_RS21790 (KFO61_21790) 4461600..4462085 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KFO61_RS21795 (KFO61_21795) 4462082..4462375 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KFO61_RS21800 (KFO61_21800) 4463020..4464396 + 1377 WP_004217775.1 cystathionine beta-synthase -
KFO61_RS21805 (KFO61_21805) 4464407..4465555 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KFO61_RS21810 (KFO61_21810) 4465556..4466467 - 912 WP_004188550.1 LysR family transcriptional regulator -
KFO61_RS21815 (KFO61_21815) 4466565..4467167 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4404578..4462626 58048
flank IS/Tn - - 4460054..4460206 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T200302 WP_004150910.1 NZ_CP073791:c4462085-4461600 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T200302 NZ_CP097808:c4007404-4007087 [Klebsiella variicola]
ATATTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCATCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT200302 WP_004150912.1 NZ_CP073791:c4462375-4462082 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT200302 NZ_CP097808:c4007094-4006807 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References