Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4621261..4622036 Replicon chromosome
Accession NZ_CP007727
Organism Klebsiella pneumoniae subsp. pneumoniae KPNIH10

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag KPNIH10_RS23325 Protein ID WP_004150910.1
Coordinates 4621261..4621746 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KPNIH10_RS23330 Protein ID WP_004150912.1
Coordinates 4621743..4622036 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH10_RS23300 4616430..4617797 - 1368 WP_004150905.1 replicative DNA helicase -
KPNIH10_RS23305 4617790..4618173 - 384 WP_004150906.1 hypothetical protein -
KPNIH10_RS23310 4618173..4619045 - 873 WP_004188557.1 ParA family protein -
KPNIH10_RS23315 4619651..4619867 - 217 Protein_4449 transposase -
KPNIH10_RS23320 4619964..4620557 - 594 WP_004188553.1 hypothetical protein -
KPNIH10_RS23325 4621261..4621746 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
KPNIH10_RS23330 4621743..4622036 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KPNIH10_RS23335 4622681..4624057 + 1377 WP_004217775.1 cystathionine beta-synthase -
KPNIH10_RS23340 4624068..4625216 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
KPNIH10_RS23345 4625217..4626128 - 912 WP_004188550.1 LysR family transcriptional regulator -
KPNIH10_RS23350 4626226..4626828 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4563040..4622287 59247
flank IS/Tn - - 4619715..4619867 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T46937 WP_004150910.1 NZ_CP007727:c4621746-4621261 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T46937 NZ_CP007727:c4621746-4621261 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT46937 WP_004150912.1 NZ_CP007727:c4622036-4621743 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT46937 NZ_CP007727:c4622036-4621743 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References