124996

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741318..742093 Replicon chromosome
Accession NZ_CP040122
Organism Klebsiella pneumoniae strain LSH-KPN148

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag FDY04_RS03660 Protein ID WP_004150910.1
Coordinates 741608..742093 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag FDY04_RS03655 Protein ID WP_004150912.1
Coordinates 741318..741611 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDY04_RS03635 736526..737128 - 603 WP_062954968.1 short chain dehydrogenase -
FDY04_RS03640 737226..738137 + 912 WP_004188550.1 LysR family transcriptional regulator -
FDY04_RS03645 738138..739286 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
FDY04_RS03650 739297..740673 - 1377 WP_004217775.1 cystathionine beta-synthase -
FDY04_RS03655 741318..741611 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
FDY04_RS03660 741608..742093 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
FDY04_RS03665 742797..743390 + 594 WP_004188553.1 hypothetical protein -
FDY04_RS03670 743487..743703 + 217 Protein_728 transposase -
FDY04_RS03685 744309..745181 + 873 WP_004188557.1 ParA family protein -
FDY04_RS03690 745181..745564 + 384 WP_004150906.1 hypothetical protein -
FDY04_RS03695 745557..746924 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743487..743639 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T124996 WP_004150910.1 NZ_CP040122:741608-742093 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T124996 NZ_CP040122:741608-742093 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT124996 WP_004150912.1 NZ_CP040122:741318-741611 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT124996 NZ_CP040122:741318-741611 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References