Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 692995..693770 Replicon chromosome
Accession NZ_CP043562
Organism Klebsiella pneumoniae strain P094-1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag F0H50_RS03510 Protein ID WP_021314147.1
Coordinates 693285..693770 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag F0H50_RS03505 Protein ID WP_004150912.1
Coordinates 692995..693288 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0H50_RS03485 688203..688805 - 603 WP_004174410.1 short chain dehydrogenase -
F0H50_RS03490 688903..689814 + 912 WP_149362165.1 LysR family transcriptional regulator -
F0H50_RS03495 689815..690963 - 1149 WP_032433524.1 PLP-dependent aspartate aminotransferase family protein -
F0H50_RS03500 690974..692350 - 1377 WP_188042481.1 pyridoxal-phosphate dependent enzyme -
F0H50_RS03505 692995..693288 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
F0H50_RS03510 693285..693770 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
F0H50_RS03515 694474..695067 + 594 WP_004188553.1 hypothetical protein -
F0H50_RS03520 695164..695380 + 217 Protein_689 transposase -
F0H50_RS03535 695895..696608 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 695164..695316 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T139013 WP_021314147.1 NZ_CP043562:693285-693770 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T139013 NZ_CP058331:c5356160-5355795 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT139013 WP_004150912.1 NZ_CP043562:692995-693288 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT139013 NZ_CP058331:c5355802-5355479 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References