Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 740647..741422 Replicon chromosome
Accession NZ_CP103605
Organism Klebsiella pneumoniae strain 4386

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A332L402
Locus tag M5S85_RS03665 Protein ID WP_021314147.1
Coordinates 740937..741422 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag M5S85_RS03660 Protein ID WP_004150912.1
Coordinates 740647..740940 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S85_RS03640 (735855) 735855..736457 - 603 WP_021440720.1 short chain dehydrogenase -
M5S85_RS03645 (736555) 736555..737466 + 912 WP_048290040.1 LysR family transcriptional regulator -
M5S85_RS03650 (737467) 737467..738615 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
M5S85_RS03655 (738626) 738626..740002 - 1377 WP_004217775.1 cystathionine beta-synthase -
M5S85_RS03660 (740647) 740647..740940 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
M5S85_RS03665 (740937) 740937..741422 + 486 WP_021314147.1 GNAT family N-acetyltransferase Toxin
M5S85_RS03670 (742126) 742126..742719 + 594 WP_004188553.1 hypothetical protein -
M5S85_RS03675 (742816) 742816..743032 + 217 Protein_721 transposase -
M5S85_RS03680 (743996) 743996..744856 + 861 WP_000158645.1 DUF6387 family protein -
M5S85_RS03685 (745072) 745072..745320 + 249 WP_000616796.1 AlpA family transcriptional regulator -
M5S85_RS03690 (745317) 745317..745628 + 312 WP_000837631.1 hypothetical protein -
M5S85_RS03695 (745643) 745643..746029 + 387 WP_001545719.1 hypothetical protein -
M5S85_RS03700 (746046) 746046..746333 + 288 WP_001291073.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 742816..742968 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17621.68 Da        Isoelectric Point: 8.5144

>T255777 WP_021314147.1 NZ_CP103605:740937-741422 [Klebsiella pneumoniae]
MILAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT255777 WP_004150912.1 NZ_CP103605:740647-740940 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332L402

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References